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KMID : 0380020030180030180
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 3 p.180 ~ p.185
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Extraction of Glycosaminoglycans from Styela clava Tunic
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Ahn Sam-Hwan
Chung Sung-Hoon
Kang Suk-Jung
Chung Tae-Sung
Choi Byeong-Dae
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Abstract
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Glycosaminoglycans (GAGS was extracted from sea squirt, Styela clava with sodium phosphate at 105 for 2 hr and deproteinized with trichloroacetic acid or hydrochloride. The GAGs obtained from tunic consist 41.7% crude carbohydrates, 31.8% crude protein, and 31.2% sulfate. It was mainly constituted of galactose, glucosamine, glucose, mannose, and glacrosamine. The prominent amino acid were phenylalanine, threonine, glutamic acid, and aspartic acid. Mineral contents was mainly constituted 3.0 mg% sodium, 1.6 mg% potassium, and 1.2 mg% phosphorus. Trichloroacetic acid, hydrochloride and 5-sulfosalicylic acid were used for deprotein of the GAGs. Effective volume for deprotein of crude GAGs were 5.0% trichloroacetic acid (w/v) and 10.0% HCI (v/v) treatment. The deproteinized GAGs contained 35.1%, 35.4% of protein and 22.0%, 18.5% of sulfate, respectively.
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KEYWORD
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styela clava, glycosaminoglycans, GAGs, polysaccharides, deprotein
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